Microscopy and Spectroscopy in Food and Plant Sciences

Wageningen Light Microscopy Centre and MicroSpectroscopy Centre will organize a three-day course on fluorescence imaging. In this course, we will provide theoretical background and give hands-on experience of state-of-the-art microscopy techniques, with support from experts in the field.

Monday May 15th

09:00 Intro and lecture “microscope construction” (Janson)
9:45 Break
10:00 Lecture “creating contrast” (Janson)
10:45 Break
11:00 Start computer practical “digital image analysis” (v.d. Krol)
12:00 Lunch
13:30 Lecture “reporter genes” (v.d. Krol)
14:15 Break
14:30 Continuation computer practical “digital image analysis” (v.d. Krol)
18:00 Dinner

Wednesday May 16th

09:00 Lecture “fluorescence microscopy and optical sectioning” (Janson)
10:00 Break
10:15 Lecture “Förster resonance energy transfer” (Borst)
10:45 Break
11:00 Lecture “Single Molecule Techniques” (Hohlbein)
12:00 Lunch
13:30 Practical 1,2,3 and 4 (depending on group assignment)
15:30 Change practical: Practical 1,2,3 and 4 (depending on group assignment)
17:30 End of day 2

Thursday May 17th

09:00 Lecture “FRET imaging applications” (Borst)
09:45 Break
10:00 Practical 1,2,3 and 4 (depending on group assignment)
12:00 Lunch
13:00 Practical 1,2,3 and 4 (depending on group assignment)
15:00 Data analysis
17:30 Evaluation and drinks

Practical 1: Microscope construction
Practical 2: Confocal (3D) imaging
Practical 3: FRET-FLIM
Practical 4: Super-resolution imaging

Registration via the EPS website.