Course 1

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Who can attend?

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Monday, 11 July 2016

Theme: Rapid sampling and quantitative analytics

09.00 Registration
09.15 Outline of the course and introduction of participants Walter van Gulik
09.45 Introduction to microbial metabolomics Walter van Gulik
10.45 Rapid sampling for quantitative metabolomics Walter van Gulik
13.30 Quantitative analysis of intracellular metabolites using LC-MS/MS Reza Seifar
14.15 Identification of compounds using high-mass-resolution GC-TOF-MS Marco Oldiges
15.15 LC-MS/MS for the quantitative analysis of product pathway intermediates Marco Oldiges
16.00 Application of LC-MS/MS for Penicillin biosynthesis pathway intermediates Reza Seifar and Amit Deshmukh
16.45 NMR for metabolomics Peter Lankhorst
17.30 Social drink and buffet

Tuesday, 12 July 2016

Theme: Quantification and validation using isotope dilution mass spectrometry
Perturbation and validation strategies
09.00 Development of quantitative analysis of metabolites using GC isotope dilution mass spectrometry Reza Seifar
09.45 Exercises: calculating concentrations from ID-MS data and validation of sampling and extraction protocols
13.45 Perturbation strategies for estimation of in-vivo kinetic properties of enzymes Walter van Gulik
14.30 Thermodynamic validation of metabolite data Aljoscha Wahl
15.30 Tackling cellular compartmentalization: application of sensor reactions Walter van Gulik
16.15 Futile cycles during penicillin production: mimic large scale on the bench using a feast/famine regime Aljoscha Wahl
17.15 Octave introduction / tour

Student Service Centre
P.O. Box 414
6700 AK Wageningen
The Netherlands

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