Colorful packages: Fluorescent proteins in complex coacervate core micelles

PhD defence

Colorful packages: Fluorescent proteins in complex coacervate core micelles

PhD candidate A (Antsje) Nolles MSc
Promotor prof.dr. WJH (Willem) van Berkel
Co-promotor dr.ing. JW (Jan Willem) Borst
dr.ir. JM (Mieke) Kleijn
Organisation Wageningen University, Laboratory of Biochemistry, Physical Chemistry and Soft Matter
Date

Wed 14 February 2018 13:30 to 15:00

Venue Auditorium, building number 362
Generaal Foulkesweg 1
362
6703 BG Wageningen
0317-483592

Summary

Proteins are the most common biomacromolecules in biological cells and are used in many applications, such as in food formulations and as therapeutic agents. For these applications, it may be desirable to protect proteins from their environment to preserve their function by packing them into nanocontainers. Therefore, we studied the encapsulation of fluorescent proteins with charged polymers and investigated formation, composition, and dynamics of the resulting nanopackages. We found that the structure and fluorescent properties of the proteins change upon encapsulation, but these changes appeared to be reversible after release of the proteins. Furthermore, we observed that the nanocontainers quickly fall apart upon addition of salt. These findings can be employed to further engineer the nanocontainers and increase their stability for use in practical applications.