PhD defence

Diving into the Argonaute world

PhD candidate P (Pilar) Bobadilla Ugarte MSc
Promotor prof.dr. D (Dolf) Weijers
Co-promotor dr. DC (Daan) Swarts
Organisation Wageningen University, Laboratory of Biochemistry

Tue 12 March 2024 16:00 to 17:30

Venue Omnia, building number 105
Hoge Steeg 2
6708 PH Wageningen
+31 (0) 317 - 484500
Room Auditorium


Argonaute proteins (Ago) are present in all domains of life, and share a common mechanism of action. Agos use nucleic acids guides to target complementary nucleic acids targets. This thesis describes the function, mechanism and structure of prokaryotic Argonautes (pAgos) and how they can be repurposed as molecular tools. We focused on pAgos that associate with other proteins to enhance the activity. Additionally, we described a pAgo from an Asgard archaeon that resembles in structure and activity eukaryotic Argonautes (eAgos). We also reviewed the common features eAgos and pAgos share, emphasizing their role in providing immunity against invaders.