PhD candidates and postdocs, maximum of 24 participants.
The MicroSpectroscopy Research Facility and the Wageningen Light Microscopy Centre of Wageningen University will organize a VLAG & EPS funded advanced practical course for postdocs and PhD candidates. The course will cover the theory behind a number of advanced microscopic and micro-spectroscopic techniques that have a wide range of applications in both the Food and Plant Sciences.
The following topics will be treated during the lectures with examples of application in Food and Plant sciences: microscopy, fluorescence spectroscopy, confocal microscopy, Förster Resonance Energy Transfer (FRET) imaging, Fluorescence Lifetime Imaging Microscopy (FLIM), image analysis, 3D Imaging, time- and spectrally-resolved fluorescence spectroscopy, Total Internal Reflection Fluorescence (TIRF) and super-resolution imaging.
The hands-on training during this course will be in the form of group assignments. For additional individual training after this course, we would like to refer you to the MicroSpectroscopy Research Facility and the Wageningen Light Microscopy Centre.
Tuesday 7 May, Forum building (nr. 102), PC room 0707
09:00 Intro and lecture “microscope construction” (Janson)
10:00 lecture “creating contrast” (Janson)
11:00 Start computer practical “digital image analysis” (Bader/deRuijter/Borst)
13:30 lecture “Fluorescent probes” (Borst)
14:30 Continuation computer practical “digital image analysis” (Bader/deRuijter/Borst)
Wednesday 8 May, Forum building (nr. 102), PC room 0707
09:00 lecture “fluorescence microscopy and optical sectioning” (Janson)
10:15 lecture “Förster resonance energy transfer” (Borst)
11:00 lecture “Single Molecule Techniques” (Hohlbein)
13:30 Practical 1,2,3 and 4 (depending on group assignment)
15:30 Change practical: Practical 1,2,3 and 4 (depending on group assignment)
17:30 End of day 2
Thursday 9 May, Radix building (nr. 107), room PC0089
09:00 lecture “FRET imaging applications” (Borst)
10:00 Practical 1,2,3 and 4 (depending on group assignment)
13:00 Practical 1,2,3 and 4 (depending on group assignment)
15:00 Data analysis
17:30 Evaluation and drinks
Practical 1: Microscope construction
Practical 2: Confocal (3D) imaging
Practical 3: FRET-FLIM
Practical 4: Super-resolution imaging
Course coordinators and faculty
- Jan Willem Borst (WUR-Biochemistry),
- Marcel Janson (WUR-Cell Biology),
- Norbert de Ruijter (WUR-Cell Biology),
- Arjen Bader (WUR-Biophysics)
- Johannes Hohlbein (WUR-Biophysics)
Date & duration
The course will be held from 7-9 May 2019.
The course language will be English.
The study load of this course is 0.9 ECTS credits.
Location & accommodation
The course will be given at Wageningen Campus. Wageningen is located 5 kms from the train station of Ede-Wageningen. This railway station can be reached by train from the airport Schiphol in about one hour.
Registration & course fee
To register please complete the electronic reply form.
The final registration date is 15 April 2019. Maximum number of participants: 24.
Registrations will be accepted in the order in which the registration form and course fee payment are received. Applicants will be informed of acceptance of their registration by E-mail. They will receive instructions for payment, and further course details.
Course fee includes downloadable lecture notes, wi-fi internet access, coffee/tea during breaks, lunches, and one course dinner, but does not cover accommodation. The course fee depends on the participant's affiliation:
|VLAG/EPS PhD candidates with an approved TSP or postdocs registered at VLAG/EPS
|| € 180
| all other PhD candidates and postdocs
|| € 360
|| € 720
- No charge until 15 April 2019
- No refund after 15 April 2019
Substitutions for participants may be made at any time until the start of the course.