PhD defence

Proteome dynamics in Lactococcus cremoris Exploring laboratory and industrial application conditions

PhD candidate Berdien B (Berdien) van Olst MSc
Promotor prof.dr. M (Michiel) Kleerebezem
Organisation Wageningen University, Host Microbe Interactomics

Tue 19 December 2023 11:00 to 12:30

Venue Omnia, building number 105
Hoge Steeg 2
6708 PH Wageningen
+31 (0) 317 - 484500
Room Auditorium


The protein repertoire (proteome) of lactic acid bacteria plays a key role in cheese quality and flavour but is influenced by various environmental stresses during the cheese production process. Therefore, it is essential to fundamentally understand how these bacteria adapt their proteome to dynamic conditions to ensure cheese quality and characteristics.

The proteome is predominantly determined by protein production that depends on genome-wide mRNA levels as well as their individual, sequence-dependent translation efficiency, which is differently orchestrated per bacterial species. Proteome adaptation under dynamic conditions is largely driven by adjustment of protein production, with a minor role for active protein degradation. Finally, to follow proteome adaptation during cheese manufacturing, we developed a procedure to determine bacterial proteomes in the complex and protein-rich cheese environment.

In conclusion, this research underpins the value of proteomics in unraveling bacterial adaptation and performance under the dynamic conditions encountered during their application in biotechnology.